Frequently Asked Questions

A synthetic polymer network held together by DNA crosslinkers. The mechanical behaviour of the gel is set by the crosslinker formulation, with no animal-derived proteins involved. Adhesion peptides are included to support cell adhesion.

Yes. DyNAtrix contains no animal- or human-derived components. It aligns with New Approach Methodologies (NAMs) and animal-free research initiatives.

No. Fully synthetic and growth factor-free. You add only what your experiment requires, with no background signalling from undefined endogenous factors.

Because the matrix actively shapes how your cells behave. A defined, consistent composition means your results reflect your experimental variable rather than lot-to-lot differences in the scaffold, which is essential for reproducibility, drug screening, and translational work.

DyNAtrix is a fully synthetic, animal-free alternative to Matrigel and other basement membrane extracts. Because its composition is chemically defined, it removes the batch-to-batch variability and undefined growth factors that come with tumour-derived matrices.

The precursors are liquid when cold, so you mix them with your cell suspension on ice, then transfer to 37°C. Gelation completes over about 60 minutes. The workflow is comparable to handling a basement membrane matrix.

Yes. The handling steps are designed to mirror a typical Matrigel workflow, so most labs adapt their existing protocols with little change.

DyNAtrix works in standard labware, including multi-well plates, microfluidic chips, and cell culture inserts.

Validated with human MSCs, iPSCs, trophoblast organoids, breast cancer tumoroids, glioblastoma and MDCK kidney cysts. Many more cell types are currently in validation, including brain organoids, gastrointestinal organoids, fibroblasts and heart.

In the Minimal Discovery Kit, stiffness can be adjusted from 1 to 100 Pa, and stress relaxation can be explored between two timescales. Contact us if you need advanced kits (stiffness 1 to 400 Pa, stress relaxation under 1s to over 100,000s).

Yes. Optically transparent and compatible with confocal, fluorescence, and light-sheet microscopy. Fix and stain inside the gel, with no need to remove cells first.

Yes. DyNAtrix is permeable and low-fouling, so you can perform antibody staining in the gel without removing cells first.

Yes. It supports extended 3D culture over days to weeks while maintaining viability and morphology. Cell culture has been tested for up to 60 days in serum-free media.

Yes. It is shear-thinning and self-healing, which makes it suitable for extrusion-based bioprinting, and it retains its mechanical properties after printing. Contact us for bioprinting-specific protocols.

DyNAtrix works in serum-free and chemically defined media.

Add DNase I to culture medium at 37°C for around 30 minutes. The gel dissolves gently, releasing cells and organoids with high viability, ready for sequencing, flow cytometry, or passaging.

As aqueous solutions, DyNAtrix is stable at 4°C for more than 6 months. No dry-ice shipping required.

DyNAtrix is currently available at 2 ml scale. You can place multiple orders. For larger volumes, please contact us at contact@dynamicmatrices.eu.

Orders are processed with the help of our distribution partner, Pelo Biotech GmbH. Interested in becoming a distributor for Dynamic Matrices in your region? Get in touch or email us at contact@dynamicmatrices.eu.

A DNA-crosslinked polymer platform for ultra-selective biomolecule capture. Instead of magnetic beads, it uses a synthetic capture material that forms a separable pellet, isolating your target without a solid interface.

A bead-free, enzyme-free rRNA depletion workflow built on the LASSO platform. It removes abundant ribosomal RNA before RNA-seq library prep, so more of your reads go toward informative RNA.

Most established workflows rely on probe hybridization followed by RNase H or DNase digestion. riboLASSO uses programmable LASSO capture chemistry and does not depend on either enzyme during depletion.

No. It is bead-free. The capture material forms a pellet recovered by centrifugation, and the depleted RNA is recovered from the supernatant. No magnetic rack is needed.

Consistent depletion efficiency above 98%, with binding capacity up to 1000 ng, and lower-bias libraries than typical bead-based methods since there is no solid-phase binding step.

Purified total RNA is the standard input. Crude lysates can also be used if sufficient nuclease inhibitors, such as EDTA, are present to protect the sample during the reaction.

riboLASSO has been used successfully across roughly 1 ng to 1000 ng of total RNA. The reaction is scalable beyond this range depending on your application.

PCR tubes are the standard format we've used and validated. Plate-based centrifuge formats have also been tested, though we recommend validating the format for your own setup before scaling up.

In principle, the depleted RNA can go directly to library prep or sequencing. Some sequencing providers prefer samples purified into water first, in which case you can use your preferred purification method; we've tested column-based purification successfully.

No, it's not strictly required. We've sequenced samples successfully without a DNase I step, and the catcher probes don't interfere with sequencing results. You may see a small additional peak on a Bioanalyzer trace, which is harmless on its own.

riboLASSO is a ready-to-use kit pre-configured for human rRNA depletion. LASSOflex is the configurable platform. You design your own catcher strand to target custom DNA or RNA instead.

LASSOflex is currently offered for custom DNA and RNA capture. Dedicated mRNA capture is under planning. For protein, antibody, or other target types, contact us to discuss a custom arrangement.

Use the catcher strand designer tool.

riboLASSO is validated for human samples. A combined human/mouse/rat kit is underway, a pan-bacteria kit is planned, and globin mRNA depletion for whole-blood samples is planned. If you'd like something else, get in touch, or try the catcher strand designer to explore your own sequence.

Reference DM-RLH-1. Materials scale with kit size:

48- and 96-reaction kits coming soon.

riboLASSO can be purchased directly via Buy. LASSOflex, being a configurable platform, goes through a request form so we can scope your target with you first.

Orders are processed with the help of our distribution partner, Pelo Biotech GmbH. Interested in becoming a distributor for Dynamic Matrices in your region? Get in touch or email us at contact@dynamicmatrices.eu.